Date: November 10, 2016 – November 11, 2016
Venue: Queensland University of Technology, Brisbane, Australia
GOBLET and the Australian Bioinformatics and Computational Biology Society (ABACBS) are delivering a hands-on RNASeq-analysis course on November 10th, 2016 at the Queensland University of Technology (QUT), Brisbane, Australia.
GOBLET member Eija Korpelainen will deliver a 1-day hands-on RNA-Seq data analysis workshop that will introduce participants to RNA-seq data analysis methods, tools and file formats. It will cover the whole workflow from quality control and alignment to quantification and differential expression analysis.
Participants will learn how to:
•check the quality of reads with FastQC and PRINSEQ
•remove bad quality data with Trimmomatic
•align RNA-seq reads to the reference genome with TopHat2
•visualize aligned reads in genomic context using the Chipster genome browser
•perform alignment level quality control using RseQC and SAMtools
•quantify expression by counting reads per genes using HTSeq
•check the experiment level quality with PCA plots and heatmaps
•analyze differential expression with DESeq2 and edgeR
•take multiple factors (including batch effects) into account in differential expression analysis