EMBL-ABR/GOBLET Hands-on training: RNA-seq data analysis

EMBL-ABR and GOBLET join forces to bring Eija Korpelainen workhsop on RNA-seq data analysis to Australia. Over one and a half days participants will be given a hands-on introduction to RNA-seq data analysis: from raw sequence reads to differentially expressed genes – introducing the theory, analysis tools and file formats involved. The program consists of alternating short lectures and hands-on exercises.

Participants will learn and practise how to:

• check the quality of reads with FastQC and PRINSEQ
• remove bad quality data with Trimmomatic
• align RNA-seq reads to the reference genome with TopHat2
• visualise aligned reads in genomic context using the Chipster genome browser
• perform alignment level quality control using RseQC and SAMtools
• quantify expression by counting reads per genes using HTSeq
• check the experiment level quality with PCA plots and heatmaps
• analyse differential expression with DESeq2 and edgeR
• take multiple factors (including batch effects) into account in differential expression analysis.

Further info and registration here.